Scientist III - in vitro primary cell culture

Scientist III - in vitro primary cell culture
Cambridge, MA
6+ month initial contract
Hourly Pay Rate: $60-63/hr (individual medical benefits available at a cost)

Orion Group is seeking a highly experienced Scientist to work in Cambridge, MA for our global Pharmaceutical client.

The scientist will be responsible to run in vitro primary cell culture in 96-well plate format, perform serial dilutions of drug molecules, perform ELISA for cytokine analysis, and perform FACS flow cytometry analysis to look at cell populations changes and cell phenotype changes. Candidates with previous mRNA LNP production and characterization, and LNP antibody conjugation are preferred.

If you have these skills, plus a passion for drug discovery and developing life-saving pharmaceuticals, read-on!

What are the top 3-5 skills, experience or education required for this position?
1. In vitro primary cell culture (human PBMC, Cyno PBMC and mouse splenocyte)
2. Serial dilutions of drug molecules and application of treatment using multichannel pipette
3. Live/Dead and antibody staining of cells and FACS flow cytometry run on BD FACS Canto II / Fortessa / LSR II and data analysis using FlowJo software
4. Good to have: mRNA LNP production and characterization
5. Good to have: LNP antibody conjugation

What is a nice to have (but not required) skill, experience, education, or certification?
1. In vitro primary cell culture (human PBMC, Cyno PBMC and mouse splenocyte)
2. Serial dilutions of drug molecules and application of treatment using multichannel pipette
3. Live/Dead and antibody staining of cells and FACS flow cytometry run on BD FACS Canto II / Fortessa / LSR II and data analysis using FlowJo software
4. Good to have: mRNA LNP production and characterization
5. Good to have: LNP antibody conjugation

What is a nice to have (but not required) skill, experience, education, or certification?
* 3-5 year experience level
* Masters preferred

Our role in supporting diversity and inclusion
As an international workforce business, we are committed to sourcing personnel that reflects the diversity and values of our client base but also that of Orion Group. We welcome the wide range of experiences and viewpoints that potential workers bring to our business and our clients, including those based on nationality, gender, culture, educational and professional backgrounds, race, ethnicity, sexual orientation, gender identity and expression, disability, and age differences, job classification and religion. In our inclusive workplace, regardless of your employment status as staff or contract, everyone is assured the right of equitable, fair and respectful treatment.